FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah6407i-030316.3510000004e079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah6407i-030316.3510000004e079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17258998
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC3853512.232754184223209TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG438550.043.53497311
GATCGGA444050.043.3526761
ACACGTC442000.043.15953413
CACACGT442600.043.15185512
ACGTCTG443550.042.9623115
CACGTCT443800.042.9482514
AGCACAC448350.042.62978410
CGTCTGA448700.042.47923716
GAGCACA452500.042.3044369
TCGGAAG456850.042.0749473
AGAGCAC456900.041.9709058
GAACTCC451600.041.9424121
GTATGCC439850.041.92597245
CAGTCAC449850.041.8357527
ACTGACC440100.041.8282832
TCCAGTC451300.041.7459625
CCAGTCA453650.041.5149526
GTCTGAA459200.041.4883117
CGTATGC445150.041.40658644
ATCGGAA466000.041.310632
Produced by FastQC (version 0.11.2)