FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l02n01_ah6407a-030316.3510000004e00f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l02n01_ah6407a-030316.3510000004e00f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15405475
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3171402.0586187702748537TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG366650.042.98418811
GATCGGA373450.042.597641
ACACGTC369950.042.5095413
CACACGT371100.042.46874612
ACGTCTG370400.042.33586515
CACGTCT372950.042.1187914
AGCACAC376250.041.96640810
GAGCACA377250.041.9457179
CGTCTGA373900.041.92753616
CCGATGT368050.041.51374433
GAACTCC375100.041.48750721
TATCTCG372050.041.3724739
GTATGCC372050.041.2747745
CAGTCAC376200.041.21682727
AGAGCAC384400.041.2123348
TCGGAAG385700.041.129083
TCCAGTC376500.041.1182525
CGATGTA370150.041.0903234
CGTATGC375150.040.92770444
CACCGAT374450.040.83357631
Produced by FastQC (version 0.11.2)