FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah841i15h-031416.3510000004e376.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah841i15h-031416.3510000004e376.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11270362
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC403269835.78144162538879TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4552050.044.7896351
TCGGAAG4554700.044.6826483
ATCGGAA4562600.044.6338842
CGGAAGA4569200.044.628534
GAGCACA4601150.044.5751089
AGAGCAC4597550.044.5674328
GCACACG4559250.044.56379711
AGCACAC4566550.044.5447910
CACACGT4558950.044.5203412
ACACGTC4566400.044.4919513
CACGTCT4569800.044.4265514
GAAGAGC4604850.044.4167826
GGAAGAG4613850.044.416145
ACGTCTG4569450.044.40336615
AAGAGCA4625400.044.354547
CGTCTGA4561250.044.3322616
GTCTGAA4602500.044.22675717
TCTGAAC4597650.044.20490618
GTATGCC4533600.044.15767345
CTGAACT4595350.044.14967719
Produced by FastQC (version 0.11.2)