FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah841i0h-031416.3510000004e1ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah841i0h-031416.3510000004e1ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888634
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC148932916.75543171200434TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1689350.044.5871411
ACACGTC1693700.044.5419513
CACACGT1691000.044.54097412
AGCACAC1694000.044.5228610
GAGCACA1711000.044.4491739
CACGTCT1695600.044.4435214
ACGTCTG1696450.044.40268715
AGAGCAC1711950.044.3824548
CGTCTGA1694850.044.31186316
TCGGAAG1704350.044.2208633
GATCGGA1708000.044.210941
GTCTGAA1710650.044.19455317
CGGAAGA1710800.044.130124
GAACTCC1688850.044.12135721
TCTGAAC1712200.044.1085618
ATCGGAA1712650.044.054682
CTGAACT1711650.044.04386519
GTATGCC1677300.044.03981445
TCCAGTC1686550.044.03236825
TGAACTC1697850.043.99878320
Produced by FastQC (version 0.11.2)