FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah84115h-031416.3510000004e666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah84115h-031416.3510000004e666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7573478
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG447500.5908777974927768TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG69800.041.32077811
AGTCACG64700.040.64864728
ACACGTC71200.040.57148413
GATCGGA73050.039.928341
CACACGT72500.039.87502712
ACGTCTG72900.039.5633915
CACGTCT73550.039.24433514
AGCACAC74750.038.70768410
GTCACGT62200.038.7016329
CGTCTGA75100.038.3744516
TCACGTG60100.038.2945530
CACGTGA59500.038.19119331
GAGCACA77550.037.6304939
CAGTCAC75050.037.62087627
CGATCTC58900.037.32634740
CCAGTCA75000.037.1360126
TCCAGTC75500.037.12846425
GAACTCC76450.037.1082621
AGAGCAC79400.036.9520768
TCGGAAG79950.036.483573
Produced by FastQC (version 0.11.2)