FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah8410h-031416.3510000004e4ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah8410h-031416.3510000004e4ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8175781
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG7585969.278575343444254TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG921400.044.20411
ACACGTC924150.044.19499213
ACGTCTG925350.044.1301215
CACGTCT924350.044.1291814
CACACGT924450.044.0995312
GATCGGA934100.044.082651
CGTCTGA924600.044.00288416
TCGGAAG934550.043.925243
GAGCACA938000.043.9027639
AGCACAC931200.043.85959210
AGAGCAC937850.043.8450138
ATCGGAA938650.043.7991682
CGGAAGA939300.043.7630274
GTCTGAA940200.043.61496417
TCGTATG884450.043.55656445
TCTGAAC937900.043.5420118
CTGAACT938550.043.33926819
CAGTCAC932650.043.30225827
CCAGTCA925700.043.24283626
GAAGAGC952100.043.242386
Produced by FastQC (version 0.11.2)