FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah840i15h-031416.3510000004e2fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah840i15h-031416.3510000004e2fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32532827
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC826399925.402031615635494TruSeq Adapter, Index 6 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC480130.14758323953832847TruSeq Adapter, Index 6 (98% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC427580.13143032420760728TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9517450.044.6746561
TCGGAAG9514750.044.558643
ATCGGAA9533600.044.533712
CGGAAGA9601400.044.261954
GCACACG9549350.044.2051311
GAGCACA9665900.044.1683169
AGCACAC9579150.044.1517810
AGAGCAC9665850.044.14488
CACACGT9567700.044.12975712
ACACGTC9587400.044.10532413
GGAAGAG9712300.044.0623055
CACGTCT9586000.044.03776614
ACGTCTG9600500.044.0117915
GAAGAGC9700800.043.9658626
CGTCTGA9587350.043.93699316
AAGAGCA9764150.043.834257
GTCTGAA9691850.043.8302517
GTATGCC9466950.043.8232945
TCTGAAC9674050.043.78555318
CTGAACT9673800.043.70388819
Produced by FastQC (version 0.11.2)