FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah840i0h-031416.3510000004e178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah840i0h-031416.3510000004e178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11970560
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC315124326.324942191509837TruSeq Adapter, Index 2 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC151410.12648531062874252TruSeq Adapter, Index 2 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3575750.044.73761
GAGCACA3602850.044.6135949
GCACACG3569050.044.6076711
AGAGCAC3602000.044.5991368
TCGGAAG3576550.044.595243
AGCACAC3575850.044.5869910
ATCGGAA3585150.044.5581552
CACACGT3574250.044.53333712
CGGAAGA3589850.044.5209054
ACACGTC3581800.044.4846113
CACGTCT3584650.044.3952714
GAAGAGC3618400.044.3587766
ACGTCTG3587350.044.3399115
GGAAGAG3631400.044.306775
CGTCTGA3577900.044.30233816
AAGAGCA3637650.044.25057
GTCTGAA3611350.044.17481617
TCTGAAC3606000.044.14365418
GAACTCC3562150.044.07956321
CTGAACT3604600.044.06343519
Produced by FastQC (version 0.11.2)