FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah84015h-031416.3510000004e5d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah84015h-031416.3510000004e5d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331884
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG6553077.865051889824678TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA815050.044.3233071
GCACACG802500.044.19033411
TCGGAAG819800.043.958533
AGCACAC809350.043.9015610
GAGCACA817700.043.8155369
ATCGGAA823500.043.8083762
AGAGCAC820100.043.736698
CACACGT810800.043.69357312
ACACGTC811700.043.6428813
CGGAAGA830000.043.509634
ACGTCTG816000.043.31639515
CACGTCT816750.043.2876414
CGTCTGA815050.043.25095416
ACGTCCG778050.042.92138332
CGTCCGC768950.042.9206433
TCTGAAC824850.042.90347318
GAACTCC811150.042.8565321
GTCTGAA828450.042.8310917
AGTCACG813950.042.79002828
GAAGAGC842600.042.7664346
Produced by FastQC (version 0.11.2)