FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah8400h-031416.3510000004e468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah8400h-031416.3510000004e468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6525022
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG5350258.199589212113TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA631050.044.4633671
GCACACG632800.044.08876411
CACACGT634950.043.94656412
ACACGTC636600.043.9142813
TCGGAAG637200.043.8936123
ATCGGAA639650.043.847632
GAGCACA642450.043.842779
AGCACAC637950.043.81244710
CACGTCT636950.043.7976414
AGAGCAC643250.043.7637568
ACGTCTG637000.043.74122215
CGTCTGA635300.043.7272516
CGGAAGA645150.043.4454274
GAACTCC634550.043.37796821
TCCAGTC631800.043.22493425
TCTGAAC646300.043.17794418
ACTCCAG636650.043.16067523
AGTTCCG608900.043.13978634
GTCTGAA648050.043.12383717
CCGTATC604750.043.06093238
Produced by FastQC (version 0.11.2)