FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah421i15h-031416.3510000004e277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah421i15h-031416.3510000004e277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10281993
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC246226623.947361177935058TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGCC132230.12860347210895787TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2804450.044.702931
GCACACG2792350.044.6312211
GAGCACA2822100.044.6039669
CACACGT2796050.044.58181812
AGCACAC2801850.044.5747710
AGAGCAC2823350.044.5698748
ATCGGAA2813200.044.5350882
ACACGTC2805500.044.52911413
TCGGAAG2809800.044.5263523
CACGTCT2803800.044.47763414
ACGTCTG2804400.044.44565615
CGGAAGA2820850.044.4451984
CGTCTGA2799950.044.38612416
GAAGAGC2836500.044.288526
GGAAGAG2846300.044.2632265
GTCTGAA2826800.044.25263217
GTATGCC2790700.044.2040145
TCTGAAC2825400.044.1989118
GAACTCC2790600.044.1530821
AAGAGCA2854350.044.1528177
Produced by FastQC (version 0.11.2)