FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah421i0h-031416.3510000004e0f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah421i0h-031416.3510000004e0f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8618463
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC5373126.2344295032652575TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG643150.043.81281711
CACACGT644500.043.75245712
ACACGTC645200.043.7264213
CACGTCT646900.043.5451814
AGCACAC648450.043.5442310
ACGTCTG646800.043.53452315
CGTCTGA647000.043.39935716
GAGCACA658900.043.342939
CATCTCG631250.043.10505739
TCCAGTC646350.043.0984125
GAACTCC648150.043.09623721
AGAGCAC662300.043.089858
CAGTCAC652000.043.02883527
GTATGCC649000.043.0274645
GTCTGAA660200.042.957617
CCAGTCA648400.042.94480526
TCTGAAC659000.042.87195618
AGTCACC650050.042.79797428
TGAACTC656850.042.76518220
ACTCCAG654200.042.70800423
Produced by FastQC (version 0.11.2)