FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah42115h-031416.3510000004e567.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah42115h-031416.3510000004e567.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10126509
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG6611656.529051620849792TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG817800.044.1868211
ACGTCTG821250.044.01785315
ACACGTC824050.043.99135613
CACACGT822650.043.95913312
CGTCTGA821600.043.8649216
CACGTCT824500.043.85798614
GATCGGA828200.043.8284571
AGCACAC828000.043.7771410
GAGCACA835300.043.7244579
CGTCCCG762650.043.6826735
ACCCGTC780150.043.63929732
AGAGCAC837750.043.580478
TCACCCG802050.043.54282430
TCGGAAG833000.043.5090033
CACCCGT784300.043.43420831
CCCGTCC779500.043.36033233
ATCGGAA837200.043.3485872
CCGTCCC775500.043.2275534
GTCTGAA839800.043.1955817
CGGAAGA841300.043.1907164
Produced by FastQC (version 0.11.2)