FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l01n01_ah4210h-031416.3510000004e3fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l01n01_ah4210h-031416.3510000004e3fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11135006
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG214505919.264102776415207TruSeq Adapter, Index 13 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG121270.10890878729656725TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2524500.044.8308141
ATCGGAA2538300.044.5166362
TCGGAAG2536100.044.4736333
GCACACG2527350.044.40225211
GAGCACA2558700.044.365829
AGCACAC2535250.044.3546210
AGAGCAC2560050.044.3274848
CACACGT2532000.044.3198212
ACACGTC2537350.044.29789713
CGGAAGA2554150.044.2578664
CACGTCT2537400.044.1935814
ACGTCTG2539350.044.16850315
CGTCTGA2535700.044.09544816
GAAGAGC2574650.044.047776
GGAAGAG2585900.043.9630055
GTCTGAA2567050.043.91187717
TCTGAAC2565000.043.8478618
AAGAGCA2594700.043.847397
GAACTCC2532350.043.8360321
TGAACTC2545800.043.7749920
Produced by FastQC (version 0.11.2)