FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr33.3520000006764e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr33.3520000006764e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7422635
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC13600.030.0390059
GGCGCCC4800.029.6973344-45
GGGCGCC13300.028.75952142-43
TCGGGGG22800.027.29646538-39
GGGGCCC7700.026.5347644-45
GGGCGGG15650.024.28861612-13
AGGGGCG14750.024.1583410-11
AAGGGGC16400.023.1724599
GGGGCGC13950.021.79933242-43
GGGCCCG4800.021.77804244-45
GGGGGGC25450.020.63060242-43
GTAGGGG14250.019.33882118-19
GGGGCCG11350.018.62951744-45
GGGGGCG18500.018.6190840-41
GCGCCCG4800.018.31335444-45
GAGCGGC15550.018.0238519
AGAGGGG29500.017.5520388
GGCGGGG22850.016.21942712-13
GGGGGCC16150.015.74050444-45
AGGGTAG18550.015.22809528-29