FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr30.35200000067617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr30.35200000067617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8449033
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC16750.036.016539
GGCGCCC5650.034.061944-45
GGGCGCC17650.032.03793742-43
GGGGCCC12400.028.35778644-45
AAGGGGC19500.028.0140679
TCGGGGG35250.027.62845438-39
GGGCGGG24400.025.99543412-13
GAGCGGC22100.025.3631219
GGGGCGC18650.024.96948242-43
AGGGGCG22800.023.65053210-11
GGGGCCG15050.022.41734344-45
GGGCCCG7500.021.85848844-45
GGGGGGC40350.021.08005742-43
GTAGGGG19000.020.75584618-19
GCGCCCG7700.020.36505144-45
CTCGGGG21650.020.29656436-37
GGGGGCG28600.018.771640-41
AGAGCGG31300.018.6667448
AGAGGGG41400.018.5875648
AGGGTAG24000.018.29731228-29