FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr27.352000000675ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr27.352000000675ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8121787
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC20250.041.288159
AAGGGGC23700.036.8813829
GGCGCCC8850.035.43760344-45
GGGCGCC26800.034.13204642-43
TCGGGGG44200.032.88978238-39
GGGGCCC18150.032.7263144-45
GAGCGGC23950.030.9426049
GGGGCGC25000.028.51133742-43
AGGGGCG28000.028.42253710-11
GGGCGGG33300.028.25186312-13
GGGCCCG10400.027.4145844-45
GATCGGG38550.026.4852771
GTAGGGG22800.026.04849218-19
GCGCCCG9200.025.0505744-45
GGGGCCG19100.023.88369644-45
ATCGGGA42250.023.8286572
CTCGGGG26050.023.3426836-37
CGGGGTG10800.022.87805614-15
TAGGGGA26250.022.80597518-19
TCGGGAG38700.022.7030033
Produced by FastQC (version 0.11.2)