FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr24.352000000675ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr24.352000000675ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15792021
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC35200.032.390319
GGGCGCC36700.032.04393842-43
GGCGCCC12150.031.48161744-45
TCGGGGG68600.030.54069138-39
AAGGGGC38950.030.2475839
GGGGCCC24450.029.92810844-45
GGGGCGC36300.026.76846142-43
GGGCGGG48750.026.65699412-13
AGGGGCG43100.025.13405810-11
GAGCGGC39300.023.813339
GTAGGGG36150.022.80412718-19
GGGCCCG14000.022.73967244-45
GGGGCCG30500.022.35576244-45
CGGGTAG22650.022.13208814-15
GGGGGGC80250.020.96017842-43
GATCGGG62700.020.2975161
CTCGGGG44200.019.99115236-37
GGGGGCG54350.019.84343540-41
GGCGGGG58850.019.65988512-13
AGGGTAG46600.019.0002428-29
Produced by FastQC (version 0.11.2)