FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr21.3520000006755c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr21.3520000006755c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10988239
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC9450.036.95725344-45
GAGGGGC24700.032.1188289
GGGCGCC29450.030.97840942-43
GGGGCCC18250.030.2022544-45
AAGGGGC28350.027.8160349
TCGGGGG53800.027.5945838-39
GGGCGGG36400.026.56453712-13
GAGCGGC30000.025.17779
GGGCCCG10950.024.51763244-45
GGGGCGC30950.024.33390442-43
GTAGGGG25650.022.41333618-19
AGGGGCG35050.022.29935610-11
GGGGCCG23450.022.08654644-45
GCGCCCG11500.020.65933444-45
GATCGGG43700.020.537161
GGGGGGC61350.020.5245942-43
CTCGGGG32450.019.90977736-37
GGCGGGG47150.019.39939912-13
TAGGGGA29950.019.11607618-19
GCGGGGG25550.018.59714714-15
Produced by FastQC (version 0.11.2)