FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr18.352000000674d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr18.352000000674d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327853
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC7100.031.45512844-45
GGGCGCC19850.030.99996642-43
GAGGGGC21150.029.6480739
GGGGCCC13450.028.96966744-45
AAGGGGC22850.027.442319
TCGGGGG37150.027.17439538-39
GGGGCGC20700.023.87343842-43
GGGCGGG26650.023.0887912-13
AGGGGCG24550.022.7394510-11
GGGCCCG7900.021.65346344-45
GCGCCCG8000.020.78882844-45
GTAGGGG22100.020.53168118-19
GAGCGGC24700.020.1941389
GGGGGGC42600.019.68734242-43
GGGGCCG16150.019.12461944-45
CGGGTAG14800.018.13981414-15
TAGGGGA25000.017.67487718-19
GGGGGCG30050.017.39145940-41
CTCGGGG24950.017.04112636-37
GGGGCGG29850.016.23489410-11
Produced by FastQC (version 0.11.2)