FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr15.35200000067483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr15.35200000067483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9754331
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC31900.047.3562249
GAGCGGC32700.039.950819
AAGGGGC35700.038.855759
GGGCGCC41300.036.0699342-43
GGCGCCC14150.035.93231244-45
GATCGGG48400.032.7681
TCGGGGG76000.032.6621438-39
GGGGCCC28150.032.15691844-45
GTAGGGG30350.030.91928118-19
AGAGCGG43200.030.1299618
GGGCGGG56500.029.30862212-13
GGGGCGC43100.028.3343842-43
AGGGGCG48250.028.2615210-11
ATCGGGA58050.027.320782
CGGGAGA57850.027.1741524
TCGGGAG51600.027.148633
GGGGCCG33000.026.78285244-45
AGGGTAG38700.026.43628328-29
CGGGTAG20050.026.18800214-15
TAGGGGA35850.025.91066718-19
Produced by FastQC (version 0.11.2)