FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr12.35200000067419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr12.35200000067419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9135308
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC19300.039.3824129
TCGGGGG43100.032.5708738-39
AAGGGGC23850.031.2716569
GGCGCCC9000.029.56452444-45
GGGGCCC16000.029.10257744-45
AGGGGCG24950.028.18250510-11
GGGCGCC30700.027.78122742-43
GGGGCGC25350.027.4590242-43
GGGCGGG31750.027.16111212-13
GATCGGG35350.026.0612221
GTAGGGG21900.026.03322218-19
GAGCGGC26350.025.7807189
CTCGGGG27900.023.15644836-37
GGGCCCG9250.022.6014544-45
GGGGCCG18700.022.2326844-45
AGAGCGG32800.021.7243868
CGGGGTG10150.021.53374514-15
TAGGGGA26650.021.2148818-19
TCCGGGG17800.020.95071238-39
GGGGGGC52300.020.8954442-43
Produced by FastQC (version 0.11.2)