FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr09.352000000673ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr09.352000000673ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8672326
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC19750.042.574799
GGCGCCC9300.036.27811444-45
AAGGGGC22650.036.0750279
TCGGGGG44350.032.77945738-39
GAGCGGC27250.030.856969
AGGGGCG25000.030.21663910-11
GGGGCCC17550.029.10721844-45
GGGCGCC31600.028.94762842-43
GTAGGGG20900.028.30428918-19
GGGGCGC25800.028.27209942-43
GATCGGG35000.027.54031
GGGCGGG30800.026.76504112-13
TCCGGGG20550.026.35524238-39
AGAGCGG31550.026.3495068
GGGCCCG8600.025.14098544-45
TGGGCGC10150.023.87665442-43
GGGGCCG20850.023.81659144-45
CTCGGGG27300.023.66690636-37
GCGCCCG10900.023.54163444-45
CCGGGGG23200.023.14005738-39
Produced by FastQC (version 0.11.2)