FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n02_tsr03.352000000672c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n02_tsr03.352000000672c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25022553
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC117900.064.1470959
AAGGGGC126900.058.811519
GAGCGGC129050.054.4449929
GATCGGG165400.053.2014351
AGAGCGG146350.048.7223138
TCGGGAG166450.046.02173
ATCGGGA195400.044.9850162
CGGGAGA211200.041.648824
TCGGGGG346750.041.04022638-39
GGCGCCC73150.040.5326944-45
AGGGGCG183100.038.4557310-11
GTAGGGG141250.037.9758218-19
GGGCGCC242250.037.31608242-43
GGGGCGC215100.035.6310942-43
CTCGGGG184950.035.39463436-37
TCCGGGG119300.035.38130638-39
GGACGGG53950.034.9447941
AGGGTAG162000.034.68509328-29
GGGGCCC143800.034.5296844-45
GAGAGCG157550.034.5241937
Produced by FastQC (version 0.11.2)