FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr36.3510000006766a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr36.3510000006766a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556732
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC376120.5736394289106219TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT12900.024.3047351
CGCGCCT7650.019.8712541
GCCGTCT102350.019.21319248-49
ATGCCGT101850.019.1211146-47
TGCCGTC103500.018.83908748-49
TATGCCG104050.018.55704546-47
TGAAAAA106100.018.46682762-63
CGCGCGT7800.017.662011
CTGCTTG116050.017.1702756-57
GAAAAAA115850.017.0561562-63
GTATGCC118450.016.50156444-45
CGTATGC117000.016.44218344-45
TTGAAAA120650.016.4169560-61
GCTTGAA123550.016.08927358-59
CGGGGCG1850.00314334815.4069351
CGCGGGT5307.2759576E-1215.2373621
CTTGAAA130400.015.17124260-61
TCGTATG129450.015.15438542-43
TGCTTGA132100.015.04791558-59
CCGGGGT5409.094947E-1214.9551891
Produced by FastQC (version 0.11.2)