FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr33.35100000067641.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr33.35100000067641.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7422635
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC341720.4603755943812406TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT13100.022.483421
CTGCTTG119250.014.45887956-57
CGCGCCT8900.014.4117181
GCCGTCT118450.014.27592748-49
TGAAAAA119800.014.07530962-63
CGCGGGG2050.006216353313.9039881
TGCCGTC121250.013.78955648-49
ATGCCGT121450.013.68862546-47
CCGGGGT5301.5807018E-913.4448941
CGCGCAT6851.8189894E-1213.176651
GAAAAAA128100.013.08916762-63
GCGGACT6551.6370905E-1113.0548891
TATGCCG127450.012.98829946-47
CGCGCGC3306.291366E-512.955991
GCTTGAA135200.012.91121358-59
TTGAAAA135800.012.41695260-61
GCGGATG21650.012.287332
CGGATGG22550.012.218083
TGCTTGA143000.012.00766858-59
CGGGCGT5152.119832E-711.9916271
Produced by FastQC (version 0.11.2)