FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr30.3510000006761a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr30.3510000006761a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8449033
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC526460.6231008921375972TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT14850.019.1946351
GCCGTCT141450.018.38513648-49
TGAAAAA142950.018.05930562-63
ATGCCGT145300.017.7181946-47
TGCCGTC145450.017.66726148-49
TATGCCG147950.017.2563646-47
CTGCTTG156550.016.97589756-57
TTGAAAA157400.016.6729860-61
GCTTGAA160000.016.46141858-59
GAAAAAA160350.016.39586662-63
GTATGCC166400.015.65701644-45
CGTATGC167050.015.38283844-45
CTTGAAA171700.015.35353460-61
TGCTTGA173350.015.08409158-59
CCGTCTT178750.014.57525850-51
TCGTATG184300.014.07190842-43
TTCTGCT206500.013.09963954-55
CGCGCGT9500.013.001841
CGCGGCT16600.012.5921441
CTCGTAT204250.012.52302742-43
Produced by FastQC (version 0.11.2)