FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr27.351000000675e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr27.351000000675e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8121787
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC725560.8933501949755639TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA170000.021.80775362-63
CTGCTTG178950.021.23465556-57
GCCGTCT180650.020.99538648-49
ATGCCGT184300.020.51514846-47
GCTTGAA184950.020.46872958-59
TGCCGTC182850.020.40507348-49
TTGAAAA188550.020.11570760-61
GAAAAAA188300.019.96583762-63
TATGCCG188650.019.8784446-47
TGCTTGA195700.019.08951258-59
CGCGGAT14250.019.0020481
CTTGAAA199800.018.74533360-61
GTATGCC207000.018.59814344-45
CCGTCTT212850.017.85267450-51
CGTATGC211950.017.82763344-45
TCGTATG230350.016.72320742-43
TTCTGCT233600.016.71421254-55
CGCGCCT10550.016.6605631
CTTCTGC244650.015.69718454-55
CGTCTTC249600.015.68084950-51
Produced by FastQC (version 0.11.2)