FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr24.351000000675af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr24.351000000675af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15792021
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1031880.6534185839798465TruSeq Adapter, Index 3 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT247800.021.55472648-49
ATGCCGT249350.021.19214646-47
TGCCGTC251050.021.12432348-49
TATGCCG252850.020.8612346-47
CGCGGAT29000.019.3302941
CTGCTTG284450.019.02810156-57
CGTATGC281350.018.7142844-45
TGAAAAA285800.018.64725962-63
GTATGCC285800.018.6057144-45
GCTTGAA310400.017.44485958-59
TCGTATG309600.017.25213442-43
TTGAAAA317900.017.10053360-61
TGCTTGA322950.016.75223558-59
GAAAAAA320300.016.74253862-63
CTTGAAA331600.016.49429560-61
CCGTCTT328850.016.343450-51
CTCGTAT356650.014.929584542-43
TTCTGCT382650.014.43039754-55
CGTCTTC399650.013.77492750-51
CTTCTGC402750.013.73380954-55
Produced by FastQC (version 0.11.2)