FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr21.3510000006755f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr21.3510000006755f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10988239
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC699920.6369719479163131TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT190650.020.5044948-49
ATGCCGT196250.019.75001546-47
TGCCGTC196350.019.54638148-49
CGCGGAT20900.019.3209631
CTGCTTG208500.018.99962256-57
TATGCCG200000.018.95220846-47
TGAAAAA203300.018.7729962-63
GCTTGAA221100.017.89539358-59
TTGAAAA221100.017.78797760-61
GTATGCC221850.017.55663944-45
TGCTTGA227250.017.18117758-59
GAAAAAA228450.017.05973862-63
CGTATGC224450.017.03582844-45
CTTGAAA233900.016.50993360-61
CCGTCTT239050.016.31320850-51
TCGTATG248150.015.71505242-43
TTCTGCT280900.014.44079454-55
CGCGGCT24500.014.1550981
CTTCTGC288150.013.78074154-55
CGTCTTC295950.013.49778850-51
Produced by FastQC (version 0.11.2)