FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr18.351000000674d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr18.351000000674d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327853
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC532920.5713211818410946TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT17750.019.5380541
GCCGTCT163800.016.68845248-49
TGAAAAA158700.016.64111762-63
CTGCTTG167950.016.44577456-57
ATGCCGT168300.016.15756846-47
TGCCGTC165850.015.96665848-49
GCTTGAA182250.015.28569158-59
GAAAAAA181750.015.01413262-63
TATGCCG176700.014.94592746-47
TTGAAAA186200.014.71908460-61
CCGGGGT7200.014.5161
TGCTTGA190500.014.32450658-59
GTATGCC197000.013.86392644-45
CTTGAAA194800.013.76447460-61
CCGTCTT205900.013.32233250-51
CGTATGC198250.013.32128844-45
CGCGCCT11100.012.8397061
CGCGGCT19650.012.8135681
GCGGGGT5956.493792E-1012.7749671
CGGGGTG5251.9956133E-812.6678312
Produced by FastQC (version 0.11.2)