FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr15.35100000067486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr15.35100000067486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9754331
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1322321.355623466130071TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT222650.029.34417348-49
TGCCGTC225950.028.7684548-49
ATGCCGT228700.028.51598746-47
TATGCCG230900.028.12086146-47
CTGCTTG246200.026.71106156-57
GTATGCC245700.026.60095844-45
TGAAAAA244950.026.3624562-63
CGTATGC248850.026.07336644-45
GCTTGAA261900.024.9918158-59
TTGAAAA264750.024.65101460-61
TCGTATG265850.024.59368342-43
TGCTTGA265450.024.53232858-59
GAAAAAA266500.024.31981862-63
CCGTCTT270250.024.2372750-51
CTTGAAA272450.023.86715560-61
CTCGTAT294000.022.1500242-43
TTCTGCT313650.021.26219454-55
CTTCTGC314650.021.05120854-55
CGCGGAT19200.021.0320631
CGTCTTC315450.020.89236550-51
Produced by FastQC (version 0.11.2)