FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr12.3510000006741c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr12.3510000006741c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9135308
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC692610.7581681975035762TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA157400.022.21046862-63
CTGCTTG181700.019.81532956-57
TTGAAAA187050.019.22307260-61
GCCGTCT185150.019.21510548-49
GAAAAAA187950.018.96675562-63
TGCCGTC192200.018.32493448-49
ATGCCGT195150.018.10877246-47
TGCTTGA200400.017.8713958-59
TATGCCG197700.017.63494346-47
CCGTCTT203850.017.32431650-51
CTTGAAA208150.017.18316760-61
GCTTGAA220450.016.66613458-59
GTATGCC222000.015.87579944-45
CGGGGGT5103.6379788E-1215.8356921
TTCTGCT234150.015.52880754-55
CGTATGC231550.015.07742644-45
GGCGCGC3950.015.03133538-39
CTTCTGC244600.014.77798754-55
CGTCTTC252350.014.37115250-51
TCTGCTT261450.013.989078556-57
Produced by FastQC (version 0.11.2)