FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr09.351000000673a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr09.351000000673a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8672326
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC795590.9173894062561763TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA161200.023.9852562-63
CTGCTTG180700.021.8176256-57
GCCGTCT179300.021.7626748-49
TGCCGTC182950.021.36743248-49
TATGCCG184800.021.05071646-47
ATGCCGT187050.020.82289346-47
GAAAAAA187300.020.66829962-63
TTGAAAA188850.020.54896760-61
TGCTTGA197300.020.18649358-59
CCGTCTT196400.020.04930150-51
GTATGCC204100.018.9903144-45
CTTGAAA210400.018.77160660-61
CGTATGC214350.018.1930144-45
GCTTGAA220750.017.92376358-59
TTCTGCT234250.016.98214754-55
CTTCTGC240150.016.81216854-55
TCGTATG234950.016.57766342-43
CGTCTTC245650.016.12632650-51
TCTGCTT256050.015.87020956-57
CGGGCCC2104.0019883E-415.8358471
Produced by FastQC (version 0.11.2)