FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr06.35100000067344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr06.35100000067344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11727106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1053040.8979538515299512TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA220750.023.4966462-63
GCCGTCT228750.023.16286748-49
TGCCGTC234400.022.44243448-49
ATGCCGT235500.022.3678646-47
TATGCCG238100.022.11363446-47
CTGCTTG242050.022.01786856-57
TTGAAAA257800.020.58962660-61
CCGTCTT257500.020.50302350-51
TGCTTGA259000.020.43004258-59
GAAAAAA257700.020.27505762-63
GTATGCC263150.020.27030244-45
CGTATGC273650.019.31027244-45
CTTGAAA281000.018.90660360-61
GCTTGAA289750.018.33565358-59
TCGTATG301600.017.77272642-43
TTCTGCT318600.017.03326854-55
CGTCTTC323700.016.70613750-51
CTTCTGC327150.016.63892454-55
CTCGTAT319050.016.49547442-43
TCTGCTT344200.015.80781256-57
Produced by FastQC (version 0.11.2)