FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l08n01_tsr03.351000000672cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l08n01_tsr03.351000000672cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25022553
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC6886852.752257133794461TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA978250.035.83611362-63
CTGCTTG1034750.034.54768456-57
GCCGTCT1042550.034.3162448-49
TGCCGTC1053000.033.7907448-49
ATGCCGT1059700.033.70494546-47
TTGAAAA1056650.033.4896160-61
TATGCCG1064650.033.41662246-47
TGCTTGA1077450.032.8849858-59
GAAAAAA1078350.032.60205562-63
CCGTCTT1092900.032.60065550-51
GTATGCC1116050.032.07338344-45
CTTGAAA1116200.031.64334160-61
GCTTGAA1133950.031.33023558-59
CGTATGC1144350.031.11832644-45
TTCTGCT1191200.030.17374654-55
TCGTATG1200600.029.83248342-43
CTTCTGC1212150.029.5425254-55
CGTCTTC1225850.029.30711450-51
CTCGTAT1247250.028.54530542-43
TCTGCTT1261200.028.44441256-57
Produced by FastQC (version 0.11.2)