FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n02_mock-2.3520000006736a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n02_mock-2.3520000006736a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31348728
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA985780.31445613997480215No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460670.14695014100731615No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334850.1068145412470962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT417950.041.0376321
CAGTGTT506150.033.8215832
AGTGTTT588500.029.2199253
GACCGCA59450.026.2043764
GTGTTTT739250.023.1871534
AGACCGC60000.018.8374773
GCATCCG84800.018.7121018
GTTTTTT939250.018.3022446
CGCATCC87250.017.8575577
TGTTTTT1019550.016.9343245
TCATTAA642500.016.66104994-95
ACCGCAT101500.016.33175
CATCCGT98350.016.2307119
GTATCAT1141100.015.91198794-95
CGTATCA1324350.015.83952794-95
TATCATT1003850.015.29500494-95
CATTAAA565000.015.25468194-95
ATCATTA849150.014.98443894-95
CCGTATC1588050.014.95212494-95
GCCGTAT1858100.014.25958594-95
Produced by FastQC (version 0.11.2)