FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n02_mock-1.352000000672ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n02_mock-1.352000000672ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38268131
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2167750.5664635150329134No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1118190.2921987488754023No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647710.16925571828945604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT676050.050.2480131
CAGTGTT773100.043.7633932
AGTGTTT865100.039.1445733
GTGTTTT1051800.032.3318374
GTTTTTT1329400.025.7154466
TGTTTTT1409350.024.1001975
GACCGCA67900.020.7748154
CGTATCA1366200.015.75774494-95
TCATTAA679500.015.71886194-95
GTATCAT1183950.015.51066394-95
CCGTATC1645950.015.28782194-95
TATCATT1051550.014.95815194-95
AGACCGC70050.014.7108923
GCCGTAT1937200.014.69151594-95
CATTAAA590850.014.494801594-95
ATCATTA916250.014.41860394-95
GCATCCG100900.013.6076588
CGCCGTA2307850.013.44167394-95
CGCATCC107450.012.7761817
GGTCTCC189700.012.2652811
Produced by FastQC (version 0.11.2)