FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n02_colcells.352000000673e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n02_colcells.352000000673e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32918743
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6938612.107799195127226No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3321861.009109005164626No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1417660.43065435396485213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT641050.048.265981
CAGTGTT733750.041.9490472
AGTGTTT804650.038.3139653
GTGTTTT1086000.028.5757984
GTTTTTT1441900.022.134256
TGTTTTT1523700.020.40855
GACCGCA82150.016.8242034
AGACCGC66200.016.5706543
CGCATCC81500.016.5528687
CCGGGGT22100.016.544732
GTATCAT796450.016.18792794-95
CCGTATC1129300.015.51467394-95
CGTATCA975400.015.43687594-95
TATCATT722100.015.04176494-95
TCATTAA471650.014.974936594-95
GCCGTAT1367200.014.80115194-95
CGCCGTA1617550.014.35945194-95
GCATCCG94200.014.1719438
CCGCATC96150.014.1290216
ATCATTA629850.014.00105694-95
Produced by FastQC (version 0.11.2)