FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n02_best-2.35200000067285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n02_best-2.35200000067285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37066524
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000120.5396027963129211No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT906580.2445818766280863No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680760.18365898027017585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT705850.044.9239851
CAGTGTT824350.038.2196272
AGTGTTT949100.033.4382133
GTGTTTT1121000.028.0435244
GTTTTTT1450750.021.7145966
GACCGCA93550.021.3231474
TGTTTTT1519150.020.647785
GCATCCG126200.016.0369458
CGCATCC125650.015.6891317
CGTATCA1320700.015.58259894-95
GTATCAT1137850.015.45813794-95
TCATTAA662850.015.37509494-95
AGACCGC92450.015.3584663
CCGTATC1583050.015.19714494-95
CATCCGT131750.015.1090099
TATCATT1025400.014.75780894-95
GCCGTAT1857350.014.55028794-95
ATCATTA887400.014.19103694-95
CATTAAA597900.013.76735394-95
ACCGCAT153750.013.6544455
Produced by FastQC (version 0.11.2)