FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n02_best-1.35200000067228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n02_best-1.35200000067228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44240123
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2258660.5105455968103886No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080590.2442556500125463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT736240.16641906714409452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT759600.048.066661
CAGTGTT881250.041.17382
AGTGTTT1010800.036.1051523
GTGTTTT1230050.029.7973234
GTTTTTT1599400.022.6336236
TGTTTTT1714950.021.223435
GACCGCA86550.018.603054
GTATCAT1325950.016.14457794-95
CGTATCA1546100.016.03684694-95
TCATTAA760950.015.56912194-95
TATCATT1174200.015.39250694-95
CCGTATC1847550.015.19469394-95
GCCGTAT2165050.014.54100294-95
ATCATTA1018150.014.37307994-95
AGACCGC89100.013.8575423
CATTAAA672500.013.8546794-95
CGCCGTA2589450.013.72384794-95
GCATCCG124450.013.0938928
TCGCCGT3099350.012.72767694-95
CGCATCC132300.012.3153367
Produced by FastQC (version 0.11.2)