FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n01_mock-2.3510000006736d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n01_mock-2.3510000006736d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31348728
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1035330.3302622039401407No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480580.15330127589227863No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460410.14686720303292689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT389600.036.851791
CAGTGTT478150.029.7246992
GACCGCA59250.027.2570884
AGTGTTT552650.025.9753593
GTGTTTT685300.021.043114
AGACCGC58850.018.484443
GCATCCG89400.017.7470518
GTTTTTT809750.017.6040366
CGCATCC91650.017.2069077
ACCGCAT103650.016.4979635
GGGGACC36100.015.79272651
GCCGTCT1358600.015.6787794-95
CCGTCTT1192100.015.45598394-95
TGTTTTT942050.015.3989925
CATCCGT103350.015.3056239
TGCCGTC1624350.014.75707294-95
ATGCCGT1893950.014.13111694-95
CGTCTTC1124800.013.8195594-95
TATGCCG2213150.013.58678594-95
CTTCTGC664850.013.29930494-95
Produced by FastQC (version 0.11.2)