FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n01_mock-1.351000000672f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n01_mock-1.351000000672f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38268131
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2215500.5789412605491498No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1254940.32793344414965026No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT876410.22901823974627872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT662800.047.7173081
CAGTGTT756950.041.468742
AGTGTTT842050.037.5935553
GTGTTTT1003350.031.4389764
GTTTTTT1174850.026.3285356
TGTTTTT1330900.023.5662575
GACCGCA70750.019.8725054
GCCGTCT1403550.015.43884394-95
TGCCGTC1691650.014.94207594-95
CCGTCTT1252050.014.83345894-95
ATGCCGT1966700.014.50918594-95
AGACCGC72500.013.8903813
GCATCCG102500.013.8566948
TATGCCG2287750.013.634706594-95
CGTCTTC1226550.013.05258394-95
CGCATCC107750.013.0047077
GTATGCC2689400.012.60690594-95
GTCTTCT1091250.012.34655894-95
CGTATGC3100000.012.11922194-95
CATCCGT120250.011.8508239
Produced by FastQC (version 0.11.2)