FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n01_colcells.351000000673e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n01_colcells.351000000673e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32918743
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6196371.8823227849252933No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3892831.182557304815679No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2349070.7135965064036619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT803700.052.812491
CAGTGTT891500.047.09752
AGTGTTT958150.044.2825473
GTGTTTT1220100.034.761284
GTTTTTT1451150.029.0766456
TGTTTTT1614050.026.2303375
GACCGCA84450.019.011024
AGACCGC64300.018.8385123
CGCATCC82750.018.2539717
CCGCATC92700.016.6533096
GCATCCG96750.015.9077038
GCCGTCT984450.015.66676494-95
CCGTCTT840000.015.45718794-95
TGCCGTC1209950.014.82561694-95
ATGCCGT1432450.014.32666894-95
TATGCCG1658900.014.23930994-95
GTATGCC1944500.013.72348794-95
ACCGCAT121000.013.5042355
CGTCTTC831050.013.3688494-95
CATCCGT118250.013.3367619
Produced by FastQC (version 0.11.2)