FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n01_best-2.35100000067288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n01_best-2.35100000067288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37066524
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2168590.5850535108174697No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT974420.26288410534529755No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT894020.2411933743773762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT657650.039.6748121
CAGTGTT762050.033.9039082
AGTGTTT874800.029.810883
GTGTTTT1023200.025.2212724
GACCGCA93800.023.0407354
GTTTTTT1230600.020.6583026
TGTTTTT1382750.018.61195
AGACCGC92950.017.6822663
GCATCCG125950.016.934048
CGCATCC132650.016.4362017
GCCGTCT1348200.015.53367394-95
CATCCGT140400.015.3603529
TGCCGTC1617900.014.9890894-95
CCGTCTT1195350.014.93111794-95
ACCGCAT159000.014.3994315
ATGCCGT1886150.014.35572394-95
GGGGACC46150.014.2064831
TATGCCG2204100.013.72334994-95
CCGCATC163350.013.5507146
CGTCTTC1161550.013.26777994-95
Produced by FastQC (version 0.11.2)