FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l07n01_best-1.3510000006722b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l07n01_best-1.3510000006722b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44240123
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2287020.5169560672333573No Hit
CCAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1306410.295299811892476No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT877400.19832675420002788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGT765700.046.3551371
CAGTGTT878750.040.0721932
AGTGTTT1003450.035.347883
GTGTTTT1202350.029.4749854
GTTTTTT1410300.024.4792866
TGTTTTT1624750.021.7423535
GACCGCA96850.019.0782014
GCCGTCT1562200.015.96745494-95
CCGTCTT1383350.015.59738594-95
TGCCGTC1876400.015.05812794-95
ATGCCGT2187400.014.30804394-95
AGACCGC96300.014.1569143
TATGCCG2565650.013.7417494-95
CGTCTTC1344650.013.41282594-95
GCATCCG138500.013.3073488
CGCATCC140100.013.0532577
GTATGCC3032750.012.96828994-95
CATCCGT144950.012.7479679
GTCTTCT1201500.012.366075594-95
CGTATGC3488950.012.28006794-95
Produced by FastQC (version 0.11.2)