FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l06n02_total_drought_1h_rep3_shoot.35200000067334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l06n02_total_drought_1h_rep3_shoot.35200000067334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108518098
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3121930.28768749706615754No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2829080.2607012150176093No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2746870.253125520132135No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2567990.23664163373007144No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA2127880.1960852649665865No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA2036040.18762216049897962No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2003480.18462173931577755No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1860170.17141564718541233No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1828610.16850737652994988No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1821160.1678208550982897No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1705540.1571664110810346No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1699500.1566098218934873No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT1594020.146889784227512No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1543890.14227027827192473No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1535760.14152109448140163No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1519860.14005590109034163No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1478160.1362132240835994No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1358200.12515884677595437No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1311060.1208148708983086No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1299230.11972473015514887No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1264810.11655290899035109No Hit
GGGGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1134110.1045088350147825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA533800.060.2133982
CGCGGAA690150.057.896263
CGGAAGT905550.056.5459145
TTGCAGT4150150.054.602129
ATTGCAG4204850.053.8956348
GCCGCGG519250.052.7734341
GCGGAAG955450.051.749694
AATCTGT1977300.050.973027
CATACGC655750.049.629929
TGACCGA420350.049.2059481
TCTGTAA2090800.048.185589
ATCTGTA2115000.047.701018
GAAGTAG5179950.047.6056147
CCGAATC622350.046.3482784
TGCTATA1714550.046.2872779
GACCGAA460600.046.0510142
AAGTAGG5391950.045.6724748
ATTGCTA1793000.044.7240947
GGAAGTA1556850.044.3553856
AGTAGGA5568100.044.2554789
Produced by FastQC (version 0.11.2)