FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l06n02_total_drought_1h_rep2_shoot.352000000672d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l06n02_total_drought_1h_rep2_shoot.352000000672d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59831212
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1021250.17068850285031834No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT968140.1618118650178773No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA947920.15843235801407465No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG920970.15392802004412012No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA877990.14674447845047833No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA866190.1447722636807023No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT783530.1309567320815764No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG738470.12342554585055038No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG729290.12189122961440259No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT679150.1135109882113035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA230100.052.9738272
CGCGGAA303900.051.8026283
CGGAAGT407650.051.0945435
TTGCAGT1600350.047.286549
ATTGCAG1638400.046.158838
TGCTATA935800.045.5052649
CATACGC314700.044.8233959
GCGGAAG448850.044.3806234
GAAGTAG2292850.044.2685057
ATTGCTA987450.043.486317
AAGTAGG2365650.042.8156478
AATCTGT898650.042.5446247
TCTGTAA926700.041.23549
AGTAGGA2468300.041.1221589
GCCGCGG249400.040.3058551
TTGCTAT1065950.040.1715628
GGTGTGC426350.040.098726
ATCTGTA954200.040.0763978
GGAAGTA748900.038.0456
TGACCGA199300.037.6199761