FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l06n02_total_drought_1h_rep1_shoot.3520000006726b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l06n02_total_drought_1h_rep1_shoot.3520000006726b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57731125
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6663241.1541850258417796No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5467270.9470229447286883No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4689240.8122550877018245No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4021860.6966536681902527No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT4000750.6929970618102453No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3406180.5900075565823462No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC3367520.5833109955851371No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3207040.5555131655584401No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3015470.5223300256144324No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2918440.5055228007422339No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2899880.5023078971698542No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2739940.4746036042083019No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2390810.41412842725652754No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2304030.3990966744542047No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG2184750.3784353760644713No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1847790.32006824741419815No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1788000.3097116157012357No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1769500.3065071051360943No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1721940.2982689147318712No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1485270.2572736976804107No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1426610.24711280093710283No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1413090.24477090997274No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1377890.23867367905960607No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1197860.20748946084109743No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC912970.1581417303057926Illumina Single End PCR Primer 1 (100% over 50bp)
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA907230.1571474659466622No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG889410.15406074279688814No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA865320.14988795039071212No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT864710.1497822881504561No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG862830.1494566405903228No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA851740.147535666419111No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA844150.1462209510034665No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT813230.14086508793999772No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA801720.13887136271811784No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA796070.1378926878698449No Hit
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC793730.13748736058755134No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG771200.133584786369571No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA705550.12221310428300158No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT634850.10996667742054222No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT623610.10801972073123467No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT615040.10653525286403133No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA603310.10450341994894434No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG596650.10334979614549343No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT590860.10234687094699782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC291450.077.430263
CCGCGGA673200.077.046282
TTGCAGT5042650.076.2409069
ATTGCAG5077950.075.73178
GCCGCGG641450.075.166271
CGCGGAA792250.073.9153063
CATACGC1472950.073.2544259
CGGAAGT913450.073.134245
AATCTGT1515900.071.331877
CGGACAT364850.071.0454565
CCGGACA344750.070.498334
GCGGAAG953850.069.135674
GAATTGC1128200.067.675176
ACATACG1608050.067.0077448
TGACCGA352800.066.946181
ACCGAAT384600.066.7027363
ATCTGTA1627500.066.481348
TCTGTAA1642050.065.956549
CCGCTGA523450.065.6594542
CCGAATC467500.065.632444
Produced by FastQC (version 0.11.2)