FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l06n01_total_drought_1h_rep3_shoot.35100000067337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l06n01_total_drought_1h_rep3_shoot.35100000067337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108518098
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG13516301.2455341780870506No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT8413920.7753471683589589No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT4228890.38969444525280933No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT3404230.3137015910470528No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT3338240.3076205777215152No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC2780480.2562226993694637No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA2499700.23034867419073268No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT2160390.19908107862340158No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1972320.18175032887141093No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG1840710.1696223979155993No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC1827340.16839034535972053No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT1668160.15372182435412754No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1533460.1413091482676005No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1460090.13454806404734443No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1421700.13101040528742036No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1196060.11021756020825209No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1161570.10703928850651252No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1116720.10290633733739049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTC1677550.050.1356545
GGTGTGC1075400.047.2799457
CCTTCTA1784000.047.1120577
AAGCCTT1806550.046.7662734
GCCTTCT1955200.043.113276
TGTGCTC1277250.041.2438629
CTTCTAT2051650.041.070388
AACAAGC2076150.040.940381
CTAGTTA3718350.036.9904143
TAATACG3737450.036.632518
AATACGT3785300.036.094219
TAGTTAA3926700.034.9631274
TTCTAGT3903100.034.896741
GTTAATA4026450.034.1187136
GGGTGTG733000.032.0647166
AGTTAAT4327300.031.8037365
TTAATAC4765400.028.7901427
TTCTATT3000750.028.3526529
TCTAGTT4882750.028.0396692
CGGTGTG376350.024.5235086
Produced by FastQC (version 0.11.2)